A techniqueoffers a promising alternative to experimental silk forming methods that rely on harsh chemicals, says Associate Professor , a researcher in the at KTH Royal Institute of Technology.
Because of its strength, which is comparable to Kevlar, and high elasticity, spider silk has gained increased attention for its potential use in medicine. Researchers are hopeful that it can be used as scaffolding for tissue repair, or for drug delivery, sensing of biomarkers and for antimicrobial coatings.
But finding a satisfactory method of giving form tohas proven elusive for those interested in such medical applications.
“Attempts have been made to imitate the spinning process using, for example, microfluidic systems, wet-spinning, and electrospinning,” Hedhammar says. “Many of these processes are quite complex to design and require harsh chemicals such as methanol to render the threads water insoluble after formation, which, for functionalized silk, would kill the bioactivity that’s needed for these medical uses.”
Resembling an incredibly small hairbrush, the surface the team used is comprised of micro-sized silicon pillars, which were rendered water repellent with an anti-wetting agent.
By placing droplets of the soluble silk proteins on top of the pillars, the team was able to achieve three distinct formations of synthetic silk: nanowires (which could be used in cancer therapy), local coatings (potentially useful in biomarker detection) and silk sheets (which could be used as cell growth scaffolds).
Similarly, to create a sheet, the scientists left a droplet to evaporate on top of the surface. The stability of the sheets was tested by submerging them in water, as well as in a cell culture medium, for a number of days.
“The sheets remained intact, without visual signs of being dissolved, confirming that the proteins had transformed into a stable silk form,” says co-author Professor KTH.of
Source : KTH Royal Institute of Technology